At the moment I am working with my Real Time RT-PCR machine. It is working overtime as it amplifies everythings that it shouldn't. When I first bought the primers, the peak was only one. I tried 3 times to confirm the conditions. Then, the problems started when I run my samples. There are smears. I had to rerun again and again to get the specific band as I can see smears on the upper lanes.
You might not understand what I am bebling about. But that's a life of a biologist who deals with molecular technology tools. As we can say that there are good days in the laboratories but long bad weeks. Cheers!
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